Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system.

doi: 10.1016/j.matbio.2021.11.002

Figure Lengend Snippet: Fig. 8. 3D volume rendering of Z stacks showing tight junctional protein Claudin-1 distribution within ALC clusters labeled with DiD for cell membrane. (a) DAPI, (b) Cldn1 labeling with Alexa Fluor 488 conjugated secondary, (c) DiD, (d) merged imaged using all three channels. Cldn1 labeling is restricted to one pole of the cell and is not detectable on the below the nucleus (green arrow in d).

Article Snippet: The following primary antibodies were used for junctional complex labeling: Alexa Fluor 555 conjugated mouse monoclonal antiE-cadherin (1:400; BD Biosciences-610181), rabbit polyclonal anti-Claudin-1 (Cldn1) (1:200; Proteintech-28674-1-AP), rabbit polyclonal anti-Par3 (1:400; Proteintech-11085-1-AP), rabbit polyclonal anti-actin (5 mg/ml; Abcam-ab8227).

Techniques: Labeling, Membrane

Journal: Chinese herbal medicines

Article Title: Self-assembled aggregations in Coptidis Rhizoma decoction dynamically regulate intestinal tissue permeability through Peyer's patch-associated immunity.

doi: 10.1016/j.chmed.2021.06.004

Figure Lengend Snippet: Fig. 5. Effects of SAA on expression of TJ proteins ZO-1, Occludin and Claudin-1 between intestinal epithelium cells, with or without participation of PPs. (A) Representative images of immunofluorescence staining TJ proteins ZO-1, Occludin and Claudin-1 (red) in intestinal villi, with nuclei stained by DAPI (blue). (B) IOD analyses of the expression levels of TJ proteins in each experimental group. Original magnification 200. The data are expressed as mean ± SD (n = 5), with data obtained from 10 randomly selected fields in immunofluorescence sections. *P < 0.05, **P < 0.01 and ***P < 0.001 vs NC group without PPs participation; #P < 0.05, ##P < 0.01 and ###P < 0.001 vs NC group with PPs participation; &P < 0.05, &&P < 0.01 and &&&P < 0.001 vs SAAH-NPPs group at the same time point.

Article Snippet: The primary rabbit antibodies against ZO-1 (PB9234), Occludin (bs-10011R) and Claudin-1 (CSB-PA001656) were purchased from Boster Biological Technology Co., Ltd (Wuhan, China), Bioss Biosynthesis Biotechnology Co., Ltd (Beijing, China) and Cusabio Biotech Co., Ltd (Wuhan, China), respectively.

Techniques: Expressing, Staining

Journal: Food Science and Biotechnology

Article Title: Promoting effect of the Maillard reaction products produced during the stir-frying process of Hordei Fructus Germinatus on the intestinal absorption of active ingredients in Hordei Fructus Germinatus

doi: 10.1007/s10068-021-00911-1

Figure Lengend Snippet: Effects of MRPs on the mRNA and protein expression levels of claudin-1 and ZO-1 in duodenal tissues. (A) The mRNA expression levels of claudin-1. (B) The mRNA expression levels of ZO-1. (C) The protein expression levels of claudin-1. (D) The protein expression levels of ZO-1. The mRNA levels of claudin-1and ZO-1 were quantified with β-actin as reference gene. The protein expression levels of claudin-1and ZO-1 were quantified with β-actin as the internal control. Values are mean ± SD (n = 6). *P < 0.05, compared with the control group; △P < 0.05, compared with the S-R-HGF group

Article Snippet: Rabbit polyclonal anti-ZO-1 antibody and Rabbit polyclonal anti-Claudin-1 antibody were provided by Beijing OriGene Technology Co., Ltd. (Beijing, China).

Techniques: Expressing